【摘要】 【目的】用伤寒杆菌内毒素在SD大鼠建立葡萄膜炎模型。【方法】将 2 0 0 μg伤寒杆菌内毒素注射于SD大鼠双足底部 ,观察眼部临床改变和组织学改变 ,并测定房水中蛋白浓度。【结果】内毒素注射 4h后出现炎症 ,以后炎症加重 ,表现为瞳孔缩小、前房明显闪辉、细胞和渗出 ,甚至出现后房积脓 ,于 2 4h发现房水蛋白浓度显著增高 ,虹膜睫状体、视网膜以及脉络膜有大量的白细胞及一定量的单核细胞和淋巴细胞浸润。【结论】伤寒杆菌内毒素在SD大鼠诱导出严重的葡萄膜炎 ,它可作为人类全葡萄膜炎的动物模型。更多还原

【Abstract】 To establish an uveitis model in Sprague Dawley rats by using Salmonella Typhimurium Lipopolysaccharide. 200 μg of Salmonella Typhimurium Lipopolysaccharide(Lps) were injected into hind footpad of Sprague Dawley rats.Clinical and histological examinations were performed at different times,and protein concentraion in the aqueous humor was determined 24 hours after Lps injection. The inflammation began 4 hours after Lps injection. Obvious inflammatory signs such as flare, cells and exudates in anterior chamber were observed thereafter.Severe inflammation as evidenced by hypopyon predominately in the posterior chamber was noted 24 hours after Lps injection. Much higher protein concentration in aqueous humor compared with that in control group was detected in the Lps treated group. Histological examination revealed massive infiltration of polymorphonuclear cells and monocytes in the iris,ciliary body, retina and choroid. [Conclusion] Uveitis with marked clinical signs is successfully induced in the Sprague Dawley rats with injection of Lps,which can act as a model for panuveitis seen in human.更多还原