【摘要】 利用抑制性消减杂交 （Suppressivesubtractionhybridization ,SSH）分离本实验室获得的一株水稻矮化突变体dwarf6 9与其野生型对照品系特光矮₂ （Oryzasativa ,TGA₂indica）间表达有差异的cDNA片段 .分别以dwarf6 9作为驱赶子 ,TGA₂为检测子 ,以及以dwarf6 9作为检测子 ,TGA₂为驱赶子建立了两个差异表达cDNA文库 ,分别代表在TGA₂和dwarf6 9中特异表达的cDNA .经反式Northern检测这两个文库共得到 10个阳性克隆更多还原

【Abstract】 The suppressive subtraction hybridization (SSH) technique was used to isolate the cDNA fragments,which showed differential expression between the rice mutant dwarf69 and wild type strain,TGA-2(Oryza sativa, indica).Two differential expressing cDNA libraries were constructed with the cDNAs of dwarf69 as driver and the cDNAs of TGA-2 as tester, vice versa. These two cDNA libraries indicated the differential expressing cDNA fragments in TGA-2 and dwarf69. By reverse Northern blots, ten positive clones were obtained.更多还原