【摘要】 目的 探讨三氧化二砷(砒霜)或ATRA对原代APL细胞或HL-60细胞影响,同时分析三氧化二砷与ATRA之间的交叉耐药性。方法 MTT法测定HL-60细胞或APL细胞的增殖性。流式细胞仪检测两类细胞的凋亡性,NBT法用于测定HL-60细胞或原代APL细胞的分化性。结果 二氧化二砷与ATRA均能明显抑制APL细胞或HL-60细胞的体外增殖,它们作用机制分别为诱导明显的凋亡或分化。体外三氧化二砷对ATRA耐药HL-60细胞具有明显的抑制增殖作用,并诱导其凋亡发生。结论 三氧化二砷与ATRA无交叉耐药性。研究结果表明,ATRA诱导的高白细胞征并非归因于分化大于凋亡。更多还原

【Abstract】 Objective To detect the effect of arsenic trioxide or ATRA on primary APL cells or HL - 60 cells and to find out the mechanism of the cross drug resistance between ATRA and arsenic trioxide. Methods The proliferation of HL - 60 cells or APL cells was measured by MTT test, the apoptosis by Flow Cytometry analysis and the differentiation of HL- 60 cells, or APL cells was detected by NBT method. Results The prolifertion of primary APL cells or HL-60 cells could be inhibited in vitro by either arsenic trioxide or ATRA, which could induce obvious apoptosis or differentiation of primary APL cells or HL - 60 cells respectively. Inhibation of proliferation or apoptosis of ATRA resistant HL-60 cells were achieved by exposure to arsenic trioxide in vitro. Conclusions The results indicated that the chief mechanism of ATRA on APL cells or HL - 60 cells was due to the induction of differentiation, and the arsenic trioxide can induce both differentiation and apoptosis of APL cells or HL - 60 cells. There was no cross drug resistance between arsenic trioxide and ATRA.更多还原