【摘要】 目的　探讨日本血吸虫组织蛋白酶BDNA疫苗 (Sj31B1N)抗生殖免疫的可能机理。 方法　用Sj31B1N免疫小鼠 ,每 2周 1次 ,每次免疫前均尾静脉采血 1次 ,作血清抗体动态变化分析 ,免疫 4次后尾蚴攻击感染。感染后 4 5天 ,计数成虫负荷和肝、肠组织内虫卵数。结果　用Sj31B1N免疫小鼠后第 2周部分小鼠血清中出现了抗体 ,随时间延长 ,出现抗体阳性组小鼠数量增多 ,抗体滴度增加。抗体阳性组小鼠与阴性组小鼠对比 ,成虫减少率为 4 0 .6% ,肝组织减卵率为 4 8.0 % ,肠组织减卵率为 4 5.4 %。结论　核酸疫苗Sj31B1N可有效地诱导小鼠产生抗体 ,并且具有免疫保护作用。核酸疫苗Sj31B1N所诱导的体液免疫可能是抗日本血吸虫生殖免疫的机理之一。更多还原

【Abstract】 Aim To search for the probable mechanism of Sj31B1N anti-fecundity immunity.Methods Mice were immunized with Sj31B1N,and collected blood in tail vein every two weeks.Sera antibody was detected in order to acquire antibody dynamics in mice and anti-fecundity immunity was determined by counting worms,and egg burden in liver and intestine on the 45th day after challenge infection with cercariae of S.japonicum.Results Part of mice immunized with Sj31B1N showed positive antibody on the fourteenth day.The mean OD value and number of positive antibody mice increased gradually.In contrast with negative antibody mice,positive antibody mice’s worm reduction rate,egg reduction rate in the liver and intestine was 40.6%,48.0% and 45.4% respectively.Conclusion Sj31B1N could induce mice to produce antibody which had immune protective function.Humoral immunity induced by Sj31B1N.probably was one of mechanism of anti-fecundity immunity.更多还原