节点文献
铁胁迫小麦根DDRT-PCR分析及ABC基因的表达差异
DDRT-PCR Analysis Under Iron-deficient Condition in Wheat Roots and Difference of ABC Gene Expression
【摘要】 采用DDRT-PCR技术比较了正常供铁和缺铁胁迫下铁高效型小麦(京-411)和铁低效型小麦(三属麦-3)的基因表达差异模式。利用从DDRT-PCR中挑选出的ABC的cDNA片段为探针进行Northern杂交,证明它的基因表达受缺铁胁迫的抑制
【Abstract】 Expression difference of gene in iron-deficiency and iron-sufficiency were compared between iron-efficient wheat cultivar Jing-411 and iron-inefficient cultivar Sanshumai-3 by differential display reverse transcript PCR (DDRT-PCR) method. Northern blotting was carried out with the probes of ATPr binding transporter (ABC) cDNA selected from DDRT-PCR products of the cultivar Jing-411 . The results demonstrated that its expression were suppressed under iron-deficiency stress.
【关键词】 小麦;
根;
铁素营养;
mRNA差别显示;
PCR;
基因表达;
【Key words】 Wheat; Roots; Iron nutrition; DDRT-PCR; Gene expression;
【Key words】 Wheat; Roots; Iron nutrition; DDRT-PCR; Gene expression;
【基金】 北京市自然科学基金资助项目(5982008);国家重点基础研究发展规划项目(973 G1999011700)
- 【文献出处】 中国农业科学 ,Scientia Agricultura Sinica , 编辑部邮箱 ,2001年03期
- 【分类号】S512.101
- 【被引频次】10
- 【下载频次】198