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水稻完全显性早熟性的发现和基因定位
Identification and Gene Mapping of Completely Dominant Earliness in Rice (Oryza sativa L.)
【摘要】 研究发现早釉核不育系6442S-7具有完全显性早熟特性,它与13个不同类型的中、迟熟品种杂交,F1抽稳期与6442S-7完全相同或十分相近。对F2和 B1F1遗传分析表明该早熟性主要受2对显性基因控制。利用F2群体和4种分子标记技术,将6442S-7携带的1个显性早熟基因定位于水稻第3染色体短臂靠近端部一侧,该基因与RAPD标记OP111.557、SSR标记 RM22、RM231、RFLP标记C515和 AFLP标记PT671的遗传距离分别为1.5、3.0、6.7、10.9和12.4cM。该基因系首次发现并定位,暂命名为Ef-cd(t)6442S-7作为完全显性早熟性种质资源,对水稻遗传改良具有重要的应用价值。
【Abstract】 The completely dominant earliness was identified in a genie male- sterile and early maturing indica line 6442S-7. F, progenies from 6442S-7 crossed with thirteen various types of medium-or late-maturing varieties, shared the same heading date as 6442S-7. The segregation of heading date in the F, and B,F, populations showed that the earliness of 6442S-7 is mainly controlled by two dominant major genes. The local linkage map of one dominant earliness gene harbored in 6442S-7 was constructed with F, population and four kinds of molecular marker techniques. The results showed that the gene was located between a RFLP marker C515 and a RAPD marker OPI11. 557 on the short arm of chromosome 3, 10. 9cM and 1. 5 cM from C515 and OPI11. 557, respectively. The genetic distances from the target gene to two SSR markers, RM22 and RM231, and one AFLP marker , PT671, were 3.0, 6.7 and 12.4 cM, respectively. This gene, being identified and mapped first, is designated tentatively as Ef-cd (t). As a new genetic resource of completely dominant earliness, 6442S-7 has splendid future in rice improvement.
【Key words】 Rice (Oryza sativa L.); Growth duration; Dominant earliness gene; Gene mapping;
- 【文献出处】 中国农业科学 ,Scientia Agricultura Sinica , 编辑部邮箱 ,2001年03期
- 【分类号】S511
- 【被引频次】64
- 【下载频次】371