Genetic mapping of telomere-associated sequences in soybean (Glycine max)

【摘要】 <正> Two telomere-associated sequences (TAS), named STAS8 and STAS10, were cloned from soybean genomic DNA using polymerase chain reaction (PCR) amplification. Southern analysis showed that they were sequences with moderate copy number in soybean genome. Sequence analysis demonstrated that STAS10 had tandemly arrayed consensus sequences of TTTAGGG and TTAGGG . The mapping of these two TAS was performed with a population of F8 re-combinant inbred line using restriction fragment length polymorphisms( RFLP). Seven out of nine polymorphic fragments were mapped to the most distal position of five linkage groups, Dla, F, G2, H and Q of soybean, and the other two loci were closely linked and mapped to two interstitial positions within linkage group Dla. The mapping of TAS in soybean is essential for completeness of a molecular genetic map of soybean.更多还原

【Abstract】 Two telomere-associated sequences (TAS), named STAS8 and STAS10, were cloned from soybean genomic DNA using polymerase chain reaction (PCR) amplification. Southern analysis showed that they were sequences with moderate copy number in soybean genome. Sequence analysis demonstrated that STAS10 had tandemly arrayed consensus sequences of TTTAGGG and TTAGGG . The mapping of these two TAS was performed with a population of F8 re-combinant inbred line using restriction fragment length polymorphisms( RFLP). Seven out of nine polymorphic fragments were mapped to the most distal position of five linkage groups, Dla, F, G2, H and Q of soybean, and the other two loci were closely linked and mapped to two interstitial positions within linkage group Dla. The mapping of TAS in soybean is essential for completeness of a molecular genetic map of soybean.更多还原