【摘要】 目的　探讨双环醇对刀豆蛋白A (ConA)引起小鼠肝脏细胞核和DNA损伤的保护作用。方法　用ConA诱发小鼠肝损伤模型 ,观察双环醇对血清转氨酶 (ALT) ,肝细胞核DNA片断化、肝细胞核内DNA对CuSO4 邻啡罗啉 维生素C H2 O2 系统损伤的敏感性以及肝脏中H2 O2 含量的作用。结果　双环醇 5 0、15 0mg/kg灌胃能保护ConA引起的小鼠肝损伤 ,降低血清中ALT水平 ,P值分别小于 0 0 5、0 0 1;减轻肝脏细胞核和DNA的损伤 ,荧光测定DNA片断化数值降低 (P <0 0 5 ,P <0 0 1) ,DNA条带出现百分率由ConA组的 10 0 %降低至 2 5 % ;防止肝细胞核DNA对CuSO4 邻啡罗啉 维生素C H2 O2 系统损伤的敏感性的降低 ,并能阻抑肝脏H2 O2 的升高 (双环醇 15 0mg/kg组 :P <0 0 5 )。双环醇在体外加药 (终浓度分别为 :4,16 ,6 4× 10 -7mol/L)也能直接保护CuSO4 邻啡罗啉 维生素C H2 O2系统对纯品DNA的损伤。结论　双环醇对ConA引起的小鼠肝细胞核DNA损伤具有明显的保护作用。更多还原

【Abstract】 Objective To study the protective effect of bicyclol on concanavalin A (Con A) induced liver nuclear DNA injury in mice. Methods Bicyclol was fed into the stomachs of 32 mice. Then Con A was injected into their caudal veins. Six, twelve, twenty four, and forty eight hours after the injection, 8 mice were killed at each time point and their blood and livers were taken to be tested. Serum alanine aminotransferase (ALT), and nuclear DNA fragmentation, DNA ladder, H 2O 2 level, and sensitivity of nuclear DNA to oxidative damage by CuSO 4 ortho phenanthrolin vitamin C H 2O 2 system in hepatocytes of mice were determined. The protective effect on damage of pure DNA by CuSO 4 ortho phenanthrolin vitamin C H 2O 2 system was determined in vitro too. Eight mice were injected with Con A only as normal controls. Nine mice were injected with Con A only as Con A controls. Results In the experimental groups, six hours after the injection of Con A, the serum ALT level increased significantly ( P <0.05), reached the peak after 12 hours ( P <0.01), and then decreased. The liver nuclear DNA fragmentation increased 12 hours after injection of Con A in the Con A control group. However, the liver nuclear DNA fragmentation was significantly less in bicyclol groups ( P <0.05 and P <0.01). The percentage of DNA ladder in the 150 mg/kg bicyclol group was 25%, significantly lower than that in Con A control group (100%). The H 2O 2 level in liver was significantly increased 12 hours after injection of Con A in the Con A control group, and remained normal in the 150 mg/kg bicyclol group. The Con A induced decrease of sensitivity of liver nuclear DNA to damage by CuSO 4 ortho phenanthrolin vitamin C H 2O 2 system was counteracted by bicyclol injection. Conclusion Bicyclol is significantly effective in protecting liver from nuclear DNA injury of hepatocytes induced by Con A in mice.更多还原