【摘要】 目的　将糖生物学理论与免疫技术相结合 ,建立一种血清学检测诊断IgA肾病 (IgAN)的新方法 ,以解决只能依靠肾活检穿刺术诊断IgAN问题。方法　应用特异性识别糖链末端的凝集素 ,并结合抗人IgA特异抗体的酶联免疫吸附法 (ELISA) ,检测血清中糖链异常的IgA的水平。结果　采用该方法 ,对各组血清 40 5nm吸光值测定结果表明 :正常对照组测定值 :0 .2 2± 0 .10 (n =15 6 ) ,IgAN确诊病人组 :0 .6 8± 0 .17(n =2 2 ) ,两者差异有显著意义 (P <0 .0 0 1) ;IgAN疑诊病人组的测定值 :0 .38± 0 15 (n =19) ,高于正常对照组。 2 2份确诊的IgAN病人血清中 ,与病理诊断的阳性符合率为 81.8%(19/ 2 2 ) ,而假阳性率仅为 1.9% (3/ 15 6 )。结论　血清中糖链异常IgA分子的凝集素亲和 ELISA检测方法 ,具有灵敏度高、特异性好的优点 ,对于IgAN的诊断具有一定的临床应用价值更多还原

【Abstract】 Objective To detect the patients with IgA nephropathy by glycobiology and immunological technique. Methods An helia aspera lectin ELISA immunoassay was developed to discriminate the aberrantly glycosylated IgA in serum from normal IgA molecules by using HAA lectin, which reacts preferentially with the GalNAc of the terminal sugar chain, and AKP conjugated specific IgA antibody. Results The results given by A 405 nm values from 156 controls, 22 patients with IgAN and 19 patients with uncertain IgAN were 0.22±0.10,0.68±0.17 and 0.38±0.15, respectively. The significance between the controls and the patients with IgAN was observed ( P <0.001). Conclusion This assay is sensitive, specific and valuable for the diagnosis of patients with IgA nephropathy.更多还原