【摘要】 目的　利用磁分离技术直接从临床粪便样品中分离人腺病毒DNA进行PCR扩增 ,提供一个方便、有效、快速的临床检验方法。方法　生物素标记的寡聚核苷酸引物同粪便中的腺病毒DNA杂交 ,随后加入结合有链亲和素的DYNABEDRM 2 80磁性珠分离DNA 寡聚核苷酸杂交体 ,这种方法捕获的腺病毒DNA能直接进行PCR扩增。结果　从收集的 10份临床粪便样品中检测出 6份腺病毒DNA阳性结果 ,证明此法能快速捕获、浓缩人粪便中的腺病毒DNA和有效地去除粪便中影响PCR扩增的抑制物 ,提高PCR扩增的敏感性。结论　该技术能快速可靠地检测临床粪便样品中的腺病毒 ,同时也适用于其他病毒性病原的临床检测更多还原

【Abstract】 Objective To establish a rapid, effective, convenient method for detecting human adenoviruses and other viral pathogens using magnetic bead separation and PCR amplification. Methods A biotinylated oligonucleotide primers were hybridized to adenovirus DNA in stool samples. Setreptavidin coated magnetic beads were then added to isolate the DNA oligonucleotide hybrid. The procedure allows for the recovery of viral DNA suitable for amplification by polymerase chain reaction. Results Ten samples collected from clinical stool were detected; six of them were positive. Results indicated that this nucleic acid separation technology is very effective in concentrating and purifying adenoviruses DNA while removing PCR inhibitors in stool samples.It also effectively increase the sensitivity of PCR amplification. Conclusion This technique can rapidly, reliably detect adenoviruses in clinical samples, and it can be used to detect other viral pathogens.更多还原