【摘要】 目的　本实验应用NO和PS治疗大鼠IIR所致肺损伤。方法　SD大鼠 45只 ,随机分为对照组 ;病变组 (肠缺血再灌注 ) ;PS组 (气道内滴入PS) ;NO组 (气道内通入NO) ;PSNO组 (联合应用PS和NO ,)。治疗后分别观察动脉氧分压 (PaO2 )、肺顺应性 (Cdyn)、阻力 (R)、湿 /干重 (W /D)比值和肺泡扩张度 (VV)。结果　①病变组PaO2 进行性降低 ,治疗后各组PaO2 不同程度提高 ,PSNO组更为明显 ;②病变组和NO组Cdyn不断降低 ,PS组和PSNO组升高 ;③W/D病变组和NO组明显升高 ,PS和PSNO组显著低于前两者。Vv在PS组和PSNO组显著高于前两组 ;④病理改变PS组和PSNO组也明显轻于病变组和NO组。结论　大鼠IIR肺功能明显受损 ;NO可显著改善氧合功能 ,但对肺部病变本身无明显作用 ;联合应用PS和NO治疗 ,疗效优于单一治疗方法更多还原

【Abstract】 Objective To use PS and nitric oxide inhalation for the treatment of the acute lung injury with intestinal ischemia reperfusion (IIR).Methods 45 SD rats were randomly allocated to 5 groups: Control group; IIR group (ischemia for 1h, reperfusion 2h); PS group (intratracheal administration of PS ); NO group (NO given by inhalation); PSNO group (both PS and NO were given). Blood gas (PaO 2), dynamic compliance (Cdyn) and resistance (R) of the respiratory system were measured respectively.2h after ventilation, Wet/Dry weight ratio (W/D) and alveolar volume density (Vv) were measured.Results (1) PaO 2 decreased significantly in the IIR group. After treatment, PaO 2 was increased, while PSNO group had highest PaO 2 ( P <0.05). (2) Cdyn in the IIR group and NO group decreased progressively. While Cdyn in the PS and PSNO group improved significantly. R in the IIR and NO group were constantly high. In the PS and PSNO group, after 120min, it was lower in PSNO group than that in the IIR group ( P <0.05). (3) The ratio of W/D in the IIR group and NO group was significantly higher, and Vv in these two groups was lower. After treatment, they all improved in PS group and PSNO group. (4) Histologic observations revealed there were lung edema, hemorrhage and neutrophil infiltration in the IIR group and NO group.These changes were much mildin the PS group and PSNO group, especially in the latter group.Conclusions NO could increased PaO 2 substantially, but showed no effect on histological changed of lung. Combination of PS therapy and inhaled NO was better than either treatment used alone.更多还原