【摘要】 目的　比较肝外胆道不同部位组织缺血 再灌注损伤的特点及差异。方法　制成豚鼠肝外胆道缺血 再灌注模型 ,观察不同部位组织缺血 3 0min再灌注 60min后 ,上皮细胞线粒体平均体积 ( V )、数密度 (NV)及组织超氧化物歧化酶 (SOD )活力。结果　缺血 3 0min后 ,胆囊 V(0 .0 942± 0 .0 10 8) μm3 及肝总管 V (0 .0 963± 0 .0 5 40 ) μm 3 较胆总管 V (0 .0 875± 0 .0 12 0 ) μm 3 明显增大 ,NV 及SOD明显减小 ;再灌注 60min后 ,胆囊 V (0 .15 76± 0 .0 5 0 8) μm 3 最大 ,NV(0 .2 15 5± 0 .0 3 4 2 ) μm-3 及SOD(12 .62 0± 2 .3 3 1)U/g最小 ,胆总管 V(0 .114 0± 0 .0 416) μm3 最小 ,NV(0 .2 5 3 9± 0 .0 3 83 ) μm-3 及SOD(13 .5 5 4± 3 .464 )U /g最大 ,肝总管 V (0 .13 72± 0 .0 42 5 )μm3 、NV(0 .2 3 2 5± 0 .0 3 5 6) μm-3 、SOD (12 .5 86± 2 .611)U /g居中。 结论　在相同实验条件下 ,豚鼠肝外胆道不同部位组织上皮细胞缺血 再灌注损伤存在差异 :缺血时 ,胆囊及肝总管的损伤较胆总管严重 ;再灌注后 ,胆囊的损伤最严重 ,肝总管次之 ,胆总管较轻更多还原

【Abstract】 Objective To comparatively study the character and difference of ischemia reperfusion injury to the tissues of common hepatic duct, common bile duct and gallbladder. Methods Ischemia reperfusion model of extrahepatic bile ducts was developed in guinea pigs to investigate bile tissue superoxide dismutase (SOD), numeral density (Nv) and mean volume () of mitochondria of epithelial cells of extrahepatic bile ducts afer ischemia reperfusion injury. Results 30 min after ischemia, the of gallbladder (0.094?2±0.010?8)?μm 3 and common hepatic duct (0.096?3±0.054?0)?μm 3 was obviously larger but Nv and SOD of them were obviously lower than those of common bile duct. After 60 min of reperfusion, the of gallbladder (0.157?6±0.050?8)?μm 3 was largest, the Nv (0.215?5±0.034?2)?μm -3 and SOD (12.620±2.331)?U/g were the lowest, the of common bile duct (0.114?0±0.041?6)?μm 3 was lowest but the Nv (0.253?9±0.038?3)?μm -3 and SOD (13.554±3.464)?U/g of them were largest. Conclusion The injury of different parts of extrahepcetic bile ducts in guinea pigs were evidently different under the same condition: after 30 min of ischemia, the injury of common hepatic duct and gallbladder were more severe than that of the common bile duct; after 60 min of reperfusion, the most severe injury among the extrahepatic bile ducts in guinea pig was gallbladder, followed by common bile duct and common bile duct.更多还原