【摘要】 目的　了解血和脑脊液白细胞介素 (IL) 2、IL 6、IL 10在猴实验性变态反应性脑脊髓炎(EAE)发病机制和病情转归中的作用 ,并进一步阐明多发性硬化 (MS)发病的免疫学机制。方法　用猴脑制成脑白质匀浆乳化剂 ,先制作猴EAE模型 10只 ;随机选另 1只猴 ,用完全福氏佐剂 (CFA)0 4ml皮下注射作对照组。然后对EAE进行临床病情分级和病程分期 ,并在不同时期收集血和脑脊液的上清液 ,用酶增敏免疫分析法测定IL 2、IL 6、IL 10的浓度 ,用配对t检验进行统计学处理。结果猴EAE发病前后血中IL 2、IL 6、IL 10有轻度变化 ,IL 2、IL 6发病后较发病前升高 ,但差异无显著意义。而猴EAE急性期第 1、3周脑脊液IL 2 (分别为 5 0± 0 8和 5 3± 1 2 )较发病前 (0 7± 0 3)明显升高 ,P值分别为 0 0 45 ,0 0 41,恢复期或慢性期IL 6、IL 10明显升高 ,P值各为 0 0 0 43,0 0 0 6 5 ,差异有显著意义。结论　IL 2正向调节免疫 ,使EAE猴的病情加重 ;IL 6、IL 10负向调节免疫 ,使EAE猴的病情减轻。此结果对今后研究EAE或MS的免疫学机制可能有重要指导作用。更多还原

【Abstract】 Objective To investigate the change of the concentration of IL-2?IL-6?IL-10 in peripheral blood and cerebral spinal fluid (CSF) in monkey’s experimental allergic encephalomyelitis (EAE),as to explaining it’s function in mechanism and prognosis of the disease. Methods First set up animal models of monkey’s EAE. Then separate the animals by their onset symptoms according to the level and the different stages of disease. At the same time survey the blood and CSF concentrations of IL-2?IL-6?IL-10, finally process the findings statistically. Results IL-2 in the first or the third week in the acute stage of EAE (5.0±0.8 and 5.3±1.2,respectively) was obviously higher than that (0.7±0.3) before the onset,P=0.045,0.041 respectively,both IL-6 and IL-10 in recuperation or chronic stage were higher significantly than before the onset,P=0.004 3,0.006 5 respectively. The findings were significant statistically. Conclusion IL-2 up-regulates immunologically,which accelerates the disease. IL-6 and IL-10 down-regulate immunologically,which reduce the disease. The findings may play an important role in studying immunological mechanism of EAE or MS in the future.更多还原