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C-myc基因扩增、p16基因变异和乙型肝炎病毒DNA整合与肝细胞癌的相关性研究

C-myc gene amplification, p16 gene alterations and HBV-DNA integration in hepatocellular carcinoma

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【作者】 邱法波王培林吴力群张建立

【Author】 QIU Fabo, WANG Peilin, WU Liqun, et al. Department of General Surgery, Affiliated Hospital of Qingdao Medical College, Qingdao 266003, China

【机构】 青岛医学院附属医院普外科!266003青岛医学院生物教研室

【摘要】 目的 探讨C myc基因扩增、p16基因变异和乙型肝炎病毒 (HBV)DNA整合与肝细胞癌 (HCC)的相关性。方法 应用差异聚合酶链反应 (d PCR)分析C myc基因扩增 ,用聚合酶链反应(PCR)和单链构象多态 (SSCP)分析p16基因变异 ,用PCR法检测整合型HBV DNA。结果  (1)HCC组外周血、癌和癌旁肝组织中C myc基因扩增阳性率分别为 48% (14/ 2 9)、45 % (13/ 2 9)和 5 2 %(15 / 2 9) ,3者间差异无显著意义 (χ2 =0 0 0 0 8,P >0 0 5 ,自由度υ =2 ) ,但明显高于肝硬化 (LC)组外周血和LC组织阳性率〔0 (0 / 12 )和 8% (1/ 12 ) ,P均小于 0 0 5〕。 (2 ) 2 9例癌组织中有 3例p16基因纯合性缺失 ,未发现点突变。 (3)正常肝、肝硬化和肝癌组织中整合型HBV DNA阳性率分别为 14%(2 / 14)、6 7% (8/ 12 )和 97% (2 8/ 2 9) ,3者间差异有显著意义 (χ2 =2 9 434 5 ,P <0 0 1)。结论  (1)HCC的发生与C myc基因扩增密切相关 ;(2 )HCC中p16基因的纯合性缺失发生频率为 10 % ;(3)HBV DNA整合与HCC的发生密切相关 ;(4 )对外周血的检测能反映肝细胞C myc基因扩增和HBV DNA整合情况。

【Abstract】 Objective To observe the roles of C-myc gene amplification, p16 gene alterations and HBV-DNA integration in the pathogenesis and progression of hepatocellular carcinoma(HCC). Methods Differential polymerase chain reaction (d-PCR) was used for defining the amplification of C-myc oncogene Alterations of exon 1 and 2 in p16 gene were analysed by PCR and single-strand conformation polymorphism (SSCP) silver staining method HBV-DNA integration was assayed by PCR Results (1)The positive rates of C-myc gene amplification of peripheral blood (PB),tumor and their adjacent liver tissues in HCC group (29 cases) were 48%(14/29), 45%(13/29) and 52%(15/29), respectively The discrepancies among them were not statistically significant, (χ2=0000*!8, P>005,υ=2), but they were significantly higher than that of PB(0%, 0/12) as well as liver cirrhosis (LC) tissues (8%, 1/12) in LC group (12 cases), respectively (2)A total of 3 homozygous deletions and no point mutations of exon 1 and 2 in p16 gene in HCC were found (3)The discrepancies of the positive rates of HBV- DNA integration among normal liver (14%, 2/14), LC(67%, 8/12) and HCC (97%, 28/29) were statistically significant (χ2=29434*!5, P<001,υ=2) Conclusions (1)C-myc gene amplifications were closely related to hepatocarcinogenesis (2)The homozygous deletions and point mutations of p16 gene were infrequently encountered in HCC (3)HBV-DNA integration was closely ralated to the progression in HCC (4)The tests of PB indicates the C-myc gene amplification and HBV-DNA integration in hepatic cells

【关键词】 癌,肝细胞癌基因
【Key words】 Carcinoma, hepatocellularOncogenes
  • 【文献出处】 中华普通外科杂志 ,Chinese Journal of General Surgery , 编辑部邮箱 ,2001年03期
  • 【分类号】R735.7
  • 【被引频次】10
  • 【下载频次】75
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