【摘要】 目的了解Kaposi肉瘤组织内人类8型疱疹病毒(HHV-8)在Kaposi肉瘤发病中的作用。方法应用荧光原位聚合酶链反应(PCR)方法对20例新疆Kaposi肉瘤组织进行HHV-8DNA检测,Kaposi肉瘤组织损害包括结节12份、斑块6份、斑片2份。同时和20份非Kaposi肉瘤组织,包括纤维瘤组织18份,血管瘤组织2份进行对照。结果20例新疆Kaposi肉瘤组织中有17例检测到HHV-8DNA(阳性率为85%),而20例非Kaposi肉瘤组织HHV-8DNA均为阴性。两组阳性率经校正χ2检验,χ2=26.2,P<0.001。实验中HHV-8DNA片段主要集中于Kaposi肉瘤组织的梭形瘤细胞和血管内皮细胞。除此之外,在Kaposi肉瘤表皮角质形成细胞内也检测到HHV-8DNA片段,但DNA片段的荧光强度明显弱于梭形瘤细胞和血管内皮细胞中的荧光强度。结论在新疆Kaposi肉瘤的发生中,HHV-8起着一定的作用,可能参与了Kaposi肉瘤的发病,但不是Kaposi肉瘤发生的唯一病因。更多还原

【Abstract】 Objective To investigate the localization of human herpesvirus (HHV-8) in lesions of Kaposi′s Sarcoma (KS) and explore the role of HHV-8 in the pathogenesis of KS in Xinjiang. Methods HHV-8 DNA was detected by fluorescence in situ polymerase chain reaction. A total of 40 paraffin-embeded specimens were studied, including 20 KS lesions (12 nodular, 6 plaque and 2 patch lesions) and 20 non-KS lesions (18 dermatofibroma and 2 hemangioma). Results HHV-8 DNA was detected in 17 (85%) of 20 KS lesions and none in non-KS lesions (P< 0.001). Positive signals for HHV-8 were demonstrated in the nuclei of spindle-shaped tumor cells and endothelial cells. In addition, HHV-8 DNA were also present in epidermal keratinocytes, however, the intensity of fluorescent signals was weaker than that in spindle-shaped tumor cells and endothelial cells. Conclusion The result of this study suggests that HHV-8 may play a role in the pathogenesis of KS.更多还原