【摘要】 目的　初步研究 1 甲基 4 苯基 1,2 ,3,6 四氢吡啶 (MPTP)致黑质多巴胺能神经元变性的分子机制。　方法　采用MPTP腹腔注射制备帕金森病褐鼠模型 ,利用mRNA差异显示技术获得在实验组及正常对照组褐鼠黑质中差异表达的基因片段。应用反Northern杂交去除假阳性 ,对真阳性的表达序列标签 (EST)进行克隆、测序及同源性比较。　结果　用 1个随机引物和锚定引物对进行差示反应 ,在实验组获得 2个黑质特异表达的EST ,其中 1个被克隆、测序 ,为 12 1bp ,与人基因abl外显子 1b有低的同源性。　结论　MPTP致黑质神经元变性可能是通过改变促神经元凋亡基因表达而起作用。更多还原

【Abstract】 Objective To study the molecular mechanism of MPTP-induced neuronal degeneration of substantia nigra in C57BL mice. Methods The C57BL mice model of Parkinson’s disease were prepared by peritoneal administration of MPTP. These differentially expressed sequenced tags (EST) were obtained with mRNA differential display technique. Reverse Northern blotting was employed to eliminate the false positive ESTs. The true positive EST were cloned and sequenced. Results One of the two specific EST in degenerative substantia nigra was sequensed to be 121 bp and showed low homology with human v-abl Abelson murine leukemia viral oncogene (abl) exon 1b. Conclusions Our data suggested that MPTP may induce altered expression of promoting apoptotic genes to cause neuronal degeneration in substantia nigra.更多还原