【摘要】 目的　研究幽门螺杆菌 (Hp)表达LewisB抗原对细菌黏附性的可能影响 ,以及Hp的babA2 基因与消化性溃疡的关系。方法　采用体外黏附分析法检测细菌的黏附性 ,聚合酶链反应法(PCR)检测Hp的babA2 基因。结果　 (1)在 47例消化性溃疡Hp株中 ,18株为babA2 基因阳性 ;而 31例非溃疡性消化不良Hp株中 ,14株为babA2 阳性 (P =0 .42 7)。 (2 )在表达LewisB抗原的 31株Hp中 ,2 4株细菌黏附阳性 ;而在不表达LewisB抗原的 47株Hp中 ,38株呈细菌黏附阳性 (P =0 .46 3)。结论　 (1)Hp的babA2 基因与消化性溃疡的发生无关。 (2 )Hp表达LewisB抗原不影响细菌的黏附 ,从而支持胃上皮LewisB是Hp的受体。更多还原

【Abstract】 Objective To investigate the possible influence of Lewis B expression in Helicobacter pylori (H.pylori) on bacterial adhesion property, and determine the relationship between babA 2 gene in H.pylori and peptic ulcer. Methods The bacterial adhesion property in a total of 78 H.pylori strains was performed using adherence assay in vitro, and the frequency of babA 2 gene was determined by polymerase chain reaction (PCR). Results Among 47 H.pylori strains isolated from peptic ulcer patients, 18 (38.3%) were positive for babA 2 gene, while babA 2 was positive in 14 (45.2%) of 31 strains isolated from non ulcer dyspepsia patients ( P =0.427). Among 31 H.pylori strains expressing Lewis B, 24 (77.4%) were positive for adherence assay, compared with 38 (80.9%) of 47 H.pylori strains without expression of Lewis B ( P =0.463). Conclusions (1) There is no association between babA 2 status in H.pylori strain and peptic ulcer in our population; (2) The expression of Lewis B in H.pylori does not interfere with bacterial adhesion property, and thus supports that the gastric epithelium is the receptor for Lewis B of H.pylori.更多还原