【摘要】 目的　通过对卵巢过度刺激综合征 (OHSS)患者血管内皮生长因子 (VEGF)水平和表达的测定 ,初步探讨VEGF在OHSS发病机理中的作用。方法　采用酶联免疫吸附试验方法对 14例中、重度OHSS患者 (OHSS组 )和同期 13例非OHSS患者 (对照组 )取卵日卵泡液、人绒毛膜促性腺激素(hCG)注射日、胚胎移植 (ET)日血清VEGF水平进行测定 ;采用免疫组织化学方法和逆转录聚合酶链反应技术检测取卵日卵巢黄素化颗粒细胞VEGF及其mRNA的表达。结果　OHSS组和对照组的卵泡液、hCG注射日血清VEGF水平分别为 (12 5 7 2± 6 48 0 )与 (1745 1± 80 2 4)ng/L ,(2 5 0 1± 10 9 5 )与(196 7± 81 7)ng/L ,两组比较 ,差异无显著性 (P >0 0 5 ) ;OHSS组ET日血清VEGF水平及注射hCG后血清VEGF水平变化程度 ,显著高于对照组 [分别为 (34 2 9± 15 8 5 )与 (2 2 2 2± 84 6 )ng/L ,(92 8±10 6 7)与 (2 5 9± 2 1 8)ng/L ,P <0 0 5 ]。OHSS组取卵日卵巢黄素化颗粒细胞VEGFmRNA的表达 ,显著高于对照组 ,VEGF/ β肌动蛋白比值分别为 1 5 0± 0 6 0、0 96± 0 5 6 (P <0 0 5 )。两组取卵日卵巢黄素化颗粒细胞有较活跃的VEGF表达。结论　VEGF在OHSS的发病机理中可能起一定的作用。更多还原

【Abstract】 Objective To explore the role of vascular endothelial growth factor (VEGF) in the pathogenesis of ovarian hyperstimulation syndrome (OHSS). Methods Fourteen moderate or severe OHSS patients and 13 non-OHSS cases were enrolled prospectively from in vitro fertilization and embryo transfer (IVF-ET) or intracytoplasmic sperm injection program. The VEGF levels of follicular fluid (FF) on the day of oocytes pick up (DOPU) and of sera on the day of human chorionic gonadotrophin (hCG) injection (DhCGI) and embryo transfer (DET) were measured by enzyme-labeled immunosorbent assay. Expression of VEGF protein and mRNA of luteinized granulosa cells obtained during OPU were detected by immunohistochemical and reverse transcription polymerase chain reaction respectively. Results The VEGF levels of FF on the DOPU and of sera on DhCGI were not significantly different between the OHSS and control groups [(1 257.2± 648.0) ng/L Vs (1 745.1± 802.4) ng/L and (250.1±109.5) ng/L Vs (196.7±81.7) ng/L, respectively, P >0.05]. However, both serum VEGF levels on DET and its increments from DhCGI to DET are significantly greater in the OHSS group as compared with the controls, so was the expression of VEGF mRNA levels of luteinized granulosa cells on DOPU. Conclusion VEGF may play a role in pathogenesis of OHSS.更多还原