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三波长分光光度法测定18-氨基酸注射液中色氨酸和酪氨酸的含量

Determination of tryptophane and tyrosine contents in 18-amino acid injection with three-wavelength spectrophotometry

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【作者】 杜珙杨戒骄朱永泉

【Author】 DU Hong, YANG Jie-Jiao, ZHU Yong-Quan (the Second Affiliated Hospital of School of Medicine of Jinan Univ ersity, Guangdong Shenzhen 518020)

【机构】 暨南大学医学院附属第二医院! 广东 深 圳 518020

【摘要】 目的:研究不经分离直接测定18-氨基酸注射液中色氨酸和酪氨酸的含量。方法:运用三波长分光光度法测定色氨酸和酪氨酸的含量。结果:三波长分光光度法简便,重现性好。结论:本方法可作为该制剂半成品及成品的质量控制。

【Abstract】 :OBJECTIVE To directly determine the contents of tryptophane and tyrosine in 18-amino ac id injection without separation. METHODS The tyrptophane and tyrosine contents were determined with three -wavelength spectrophotometry. RESULTS The average recovery rate of tryptophane was 99.99%,RS D=0.32%,and the average rcovery rate of tyrosine was 100.1%,RSD= 0.62%.CONCLUSIONS This method is simple and repeatable, and can be used as the quality contro l method for the finished products or semi-finished products of this preparatio n.

  • 【文献出处】 中国医院药学杂志 ,Chinese Journal of Hospital Pharmacy , 编辑部邮箱 ,2001年09期
  • 【分类号】R927.2
  • 【被引频次】8
  • 【下载频次】177
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