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红蛋巢菌属生防菌的筛选及其对油菜菌核病的防治潜能
Screening for Nidula species as novel biological control agents to Sclerotinia sclerotiorum
【摘要】 通过对采自云南的红蛋巢菌属 (Nidula)鸟巢菌标本进行平板筛选 ,获得BNF71等 11个对核盘菌具有较强抑制作用的菌株。温室防病试验表明 ,BNF71和BNF137菌株的代谢液对核盘菌引起的油菜菌核病具良好防治效果 ,发病率防效分别达 98.8%和 86 .1% ,病斑扩展抑制率分别达 99.7%和 97.9%。BNF71活菌接种对油菜出苗的防病效果达 85 .2 %。除抑制核盘菌外 ,BNF71对Aspergillusniger、A .flavus、Pythi umultimum、Rhizoctoniasolani和Drechslerasorokiniana等重要的植物病原菌亦有不同程度的抑制作用。Nidula菌株具有较高的木聚糖酶活性 ,BNF134具有显著的外几丁质酶活性
【Abstract】 out of 39 isolates of Nidula spp from Yunnan province showed clear inhibition zone in dual culture test with S.sclerotiorum . Greenhouse trials with cultural filtrate of BNF71 and BNF137 showed reduction disease incidence by 98.8% and 86.1%, inhibition lesion enlargement by 99.7% and 97.9%, respectively. Wheat bran culture preparation of BNF71 was also effective in suppression of rapeseed seedling stem rot caused by S.sclerotiorum , with disease decreased by 85.2%. In addition to the highly antagonism against S.sclerotiorum , BNF71 and BNF162 were able to inhibit Aspergillus niger, A.flavus, Pythium ultimum, Rhizoctonia solani and Drechslera sorokiniana in vitro . Nidula isolates possessed high xylanase activity, and BNF134 exhibited significant exo-chitinase activity.
【Key words】 Nidula; Rapeseed ( Brassica napus L.); Biological control; Sclerotinia sclerotiorum;
- 【文献出处】 中国油料作物学报 ,Chinese Journal of Oil Crop Scieves , 编辑部邮箱 ,2001年02期
- 【分类号】S435.654
- 【被引频次】25
- 【下载频次】128