【摘要】 根据伪狂犬病病毒闽A株gE基因表位抗原编码区的序列与两种真核表达载体pPICZaA、pAcGP6 7A的序列与特性分别设计了两对PCR引物。通过PCR方法扩增到了两端具不同酶切位点的gE基因表位抗原编码片段。将这 2个片段分别克隆到pPICZaA与pAcGP6 7A载体 ,转化大肠杆菌TOP 10菌株及XL1_Blue菌株 ,获得了含伪狂犬病病毒闽A株gE基因表位抗原编码区的重组质粒pPICZaA_FS与pAcGP6 7A_FS。序列测定结果显示两个重组质粒中插入片段的大小与方向均正确。更多还原

【Abstract】 The gE gene epitope encoding area of pseudorabies virus Fa strain was amplified by PCR from template plasmid pMD18T_FS.Then the PCR products were cloned into pPICZaA and pAcGP67A vector respectively.The recombinant plasmids pPICZaA_FS and pAcGP67A_FS were identified by double restriction endonuclease digestion and DNA sequence analysis.The sequencing results showed that the recombinant plasmid contained the gE gene epitope encoding area of PRV Fa strain.更多还原