【摘要】 在RK 1 3细胞上培养的马动脉炎病毒 (Equineviralarteritis ,EAV)在 4℃、室温和 37℃条件下 ,用多种动物的红细胞进行血凝试验 ,病毒在不同温度下均可凝集小鼠和鸡的红细胞 ,但不能凝集牛、绵羊、山羊。马、猪、豚鼠、仓鼠、兔及鹅的红细胞。将病毒用吐温 80和乙醚处理后 ,血凝活力会显著增强 ,其HA效价比未经处理的病毒高 4— 8倍 ,且血凝像更加清晰。处理病毒的最适条件是在冰浴状态下用终浓度 0 .6— 1 .2 5mL/L吐温 80振荡处理 1 5— 6 0min ,再加 1 /2体积的乙醚振荡作用 5— 1 5min。更多还原

【Abstract】 The hemagglutination of equine arteritis virus (EAV) Burucys strain cultured in RK 13 was cell tested with erythricytes from many varieties of animals at 4 ℃, room tenperation and 37 ℃.HA was observed at all temperatures with mouse and chicken erythrocytes but not with those of cattle, sheep, goat, horse, pig, guinea pig, rabbit and goose. The HA activity was enhanced after treatment of virus material with Tween 80 and ether. Compared with the treated EAV with Tween 80/ether and the untreated virus, the HA titers of treated group were 4—8 times higher than the untreated and the HI pattern of the treated virus were more obvious. The optimum conditions were as follows: pretreatment of virus materials with Tween 80 at a final concentration of 0.6—1.25 mL/L for 15—60 min, and treatment with ether at a concentration of 1/2 volume for 5—15 min in ice bath with continuous shakings.更多还原