【摘要】 以肝片吸虫的分泌 排泄物作为抗原 (ES抗原 ) ,建立水牛肝片吸虫病间接ELISA诊断方法 ,并用此方法与粪便检查法检测江苏和安徽 2省 10县 (市 ) 3 0 2户的 3 0 7头水牛的血清和粪便 ;间接ELISA诊断法的最佳工作条件 ,每孔包被0 .2 μg抗原 ,血清稀释度为 1∶960 ,二抗稀释度为 1∶60 0。结果 :粪便检查出虫卵的阳性水牛 43头 ,阳性率为 14 .0 1% ,其中安徽省为 11.61% ( 18/15 5 ) ,江苏省为 16.45 % ( 2 5 /15 2 ) ;用间接ELISA法检测 2 99头水牛血清 ,阳性 87头 ,阳性率为2 9.19% ,其中安徽省为 2 3 .5 3 % ,江苏省为 3 4 .92 % ,两种方法的符合率为 86.0 5 %。更多还原

【Abstract】 The indirect enzyme linked immunosorbent assay (ELISA) was set up using adult fluke exretory secretory products as antigen. The Fasciola hepatica infection of 302 water buffaloes in 10 conties in Jiangsu and Anhui province were analyzed by ELISA and corpological examination. The results showed the optimmal working condition of ELISA were that antigen concentration was 0.2 μg/well, serum and horseradish peroxdase conjugated rabbit anti water buffalo IgG DILUTED 1∶960 and 1∶600, respectively. 43 water buffaloes were positive by corological examination. Positive rate was 14.01%. Positive reate of water buffaloes in Anhui and Jiangsu province was 11.06% (18/115) and 16.48% (25/152), respectively. The serum samples of 299 water buffloes were examined by ELISA. The results showed that positive rate was 29.19% (87/299), 23.53% and 34.59% in Anhui and Jiangsu provinces, respective. The agreement rate betwen ELISA and feces examination was 86.05%.更多还原