【摘要】 用 MDCC－ MSB1细胞增殖鸡传染性贫血病毒 (CAV)CuX－ 1毒株制备抗原，建立了检测 CAV抗体的间接免疫荧光的方法。应用该方法检测 CAV基因工程亚单位苗首免、二免和三免后鸡体内的 CAV抗体，结果表明首免一个月后，鸡血清中 CAV抗体的滴度很低或几乎测不到抗体；二免一个月后，鸡血清中可测到较高的 CAV抗体；三免一个月后，鸡血清中的 CAV抗体滴度更高。另外，应用此方法调查了某禽场 3个批次鸡体内的 CAV抗体，其中 32日龄黄鸡和 1日龄乌骨鸡体内的 CAV抗体为阴性，而 1日龄黄鸡体内的 CAV抗体为阳性。更多还原

【Abstract】 ： An indirect immunofluorescent antibody technique(IIFA)for detecting the antibody to chicken anemia virus(CAV)was developed using CAV(CuX－ 1)－ infected cell smears.Serum antibody to CAV was detected in chickens which inoculated CAV subunit vaccine.The result showed that the titre of antibody to CAV was very low,higher and more higher 30 days post the first,the second and the third inoculation respectively.Otherwise,the serum antibody to CAV in three batches of chickens in one farm was also detected using this method.The result indicated that serum antibody to CAV in 32 day－ old yellow chickens and 1 day－ old silk fowl was negitive,while 1 day－ old yellow chickens that was positive.更多还原