【摘要】 为了解上海市健康人群麻疹抗体状况 ,在上海市黄浦区和金山区对 5 0 5名健康人采血 ,用血球疑集抑制 (HI)试验和酶联免疫吸附试验 (ELISA)平行检测。结果HI试验的阳性率为 99 0 1% ,几何平均滴度 (GMT)为 1∶2 6 0 0 ;ELISA试验则为 93 6 6 %和 1∶130 3 46。HI抗体滴度高者ELISA -IgG抗体滴度也高 ,两种方法的相关系数 (r)为 0 5 8(P <0 0 0 0 5 )。但HI试验检测阴性 5份 ,ELISA试验检测阴性 32份 ,两种方法的阳性符合率为94 4%。两种方法的特点是 :HI试验检测的是麻疹血凝素抗体 ,包括IgM和IgG抗体 ;ELISA试验检测的是麻疹病毒全抗体 ,包括血凝素、血溶素的IgG和核蛋白等。分析了ELISA试验检测≥ 7岁 5个年龄组抗体阳性率低于HI试验的影响因素 ,以及金山区各年龄组麻疹抗体高的原因。更多还原

【Abstract】 In order to compare the hemagglutination inhibition (HI) test and the ELISA in detecting measles antibody, we randomly selected 505 healthy persons aged 0-20 years old from Pudong and Jinshan Districts of Shanghai. The results showed that these two tests were significantly correlated (r=0.58, P <0.0005), as indicated by the positive rates of measles antibody 99.01% and 93.66%, the geometric titers (GMT) 1∶26.0 and 1∶1 303.46 for HI test and ELISA respectively. The higher ELISA-IgG titers were parallel with the liters of HI. There were 5 blood specimens tested as negative (1∶2) by HI test while 32 were negative (<1∶250) by ELISA, the coincident rate of the two tests was 94.4%. Detection of specific antibodies of measles by the two tests is different, HI test is for hemagglutinin antibody involving IgM and IgG and ELISA is for whole antibody of measles, including hemagglutinin-IgG, hemolysin-IgG, nuclear protein and so on. Antibody levels of measles detected with ELISA in persons aged ≥7 years old were lower since the IgG antibody of measles had decreased. Antibody levels detected with both HI test and ELISA in Jinshan District was higher, this might contribute to the boosted measles vaccination to the students in primary and middle schools in 1993 and 1997.更多还原