【摘要】 目的　观察血管紧张素Ⅱ (AngⅡ )、苯那普利 (Benazepril,Be na)、洛沙坦 (Losartan)对培养的SHR和WKY大鼠心肌成纤维细胞 (CFb)增殖及合成胶原的影响。方法　采用组织块贴壁法培养CFb ,分别测定AngⅡ、Bena干预下CFb的增殖情况 ,AngⅡ、Bena和Losartan干预下CFb的3H 脯氨酸 (3H Proline)掺入量。结果　各种浓度的AngⅡ (10 -10 mol～ 10 -6mol)和Bena(10 -9mol～ 10 -5mol)对CFb的细胞数显著增殖无影响 ,AngⅡ呈浓度依赖性促进CFb的3H Proline掺入量 ,Bena抑制CFb的3H Proline掺入量 ,Losartan呈浓度依赖性抑制 10 -7molAngⅡ诱导的CFb的胶原合成。结论　AngⅡ和Bena不影响CFb的增殖 ,AngⅡ对CFb的胶原合成有促进作用 ,Bena抑制CFb胶原合成 ,Losartan可拮抗AngⅡ的作用更多还原

【Abstract】 Objective\ To investigate the effects of angiotensinⅡ(AngⅡ), Benazepril(Bena) and Losartan on proliferation and collagen synthesis of cultured rat cardiac fibroblasts(CFb) derived from SHR (CFb\-\{SHR\}) and WKY (CFb\-\{WKY\}). Methods\ CFb derived from 12\|week\|old SHR and WKY was cultured by outgrowth of tissue block. Cell proliferation of CFb was determined by direct cell counting .\{\}\+3H\|Proline incorporation of CFb was measured after incubation with AngⅡ,Bena and Losartan. Results\ In serum free(0 4%FCS) medium, cell number of CFb derived from SHR and WKY were not influenced by AngⅡ(10\+\{-10\}mol～10\+\{-6\}mol)and Bena(10\+\{-9\}mol～10\+\{-5\}mol). Increased \{\}\+3H\|proline incorporation was induced by AngⅡ in a concentration dependent manner. Benazepril caused an decrease in \{\}\+3H\|Proline incorporation in CFb derived from SHR and WKY. The increased collagen synthesis induced by AngⅡ(10\+\{-7\}mol) was inhibited by Losartan(10\+\{-10\}mol～10\+\{-6\}mol) in a concentration dependent manner. Conclusion\ Proliferation of CFb were not influenced by AngⅡ and Bena. Collagen synthesis of CFb was promoted by AngⅡ and inhibited by Bena. Collagen synthesis of CFb induced by AngⅡ was inhibited by losartan.更多还原