【摘要】 目的　早期半定量检测HIV - 1nefRNA。方法　以nef基因引物nef6 ,nef7和β-actin基因引物BA1,BA4 在同一反应体系中对标本进行逆转录聚合酶链反应 ,用BIO - 1D软件对条带密度进行分析 ,半定量测定HIV - 1nefRNA水平。结果　所试 7例实验标本均扩增出HIV - 1nef和 β -actin的基因产物。且通过BIO - 1D软件分析条带密度 ,判断艾滋病病毒感染情况。结论　该方法灵敏 ,简便 ,经济 ,是检测HIV早期复制和研究HIV致病机理的一种理想手段更多还原

【Abstract】 Objective Early semi-quantitative detection of HIV-1 nef RAN level.Methods In the same reaction,the primers nef 6 and nef 7 for nef gene and the primers BA 1,BA 4 for gene β-actin were used to detect the samples with reverse polymerase chain rection.BIO-1D software was used to analyze the density of the bands and get the semi-quantitition of HIV-1 nef RNA level.Results The amplified gene production of HIV-1 nef and β-actin were showed in seven samples,and the difference among 7 samples were obtained by the analysis of BIO-1D software.Conclusion This method was sensitive,simple and economic,and was one of the optimal method in study of HIV-1 early replication and HIV pathogenesis.更多还原