【摘要】 目的　建立红树莓的再生系统 ,短期内获得大量优质种苗。方法　利用红树莓的茎尖和茎段作为外植体 ,采用正交实验设计筛选培养基。结果　筛选出了红树莓愈伤组织、不定芽及不定根的最佳诱导培养基 ,在实验室建立了程序简单、重复性好的再生系统 ,实现了红树莓组培苗的快速繁殖。结论　用 MS培养基作为基本培养基 ,附加 BA 0 .2 m g/ L +NAA 1.0～ 1.5 m g/ L适用于愈伤组织的诱导 ;附加 BA 1mg/ L +NAA 0 .1～ 0 .2 m g/ L +GA36～ 8mg/ L+CH30 0 m g/ L 适于芽的诱导 ;附加 BA1m g/ L+NAA0 .1mg/ L+GA32 m g/ L 适于芽的繁殖 ;附加IBA 0 .1m g/ L +NAA 0 .5 m g/ L适于根的诱导。更多还原

【Abstract】 Object\ To establish a plantlet regeneration system of Rubus idaeus L for the purpose to obtain a large number of high quality seedling in a short time Methods\ Stem apex and part of the stem were used as the explant and the optimal culture media and conditions were selected by orthogonal design Results\ An optimum culture medium for the induction of callus, adventitious bud and root was obtained which can be carried out in the laboratory with comparative ease and good repeatability Conclusion\ A basic medium + BA 0 2 mg/L+NAA 1 0～1 5 mg/L was most suitable for the induction of callus; a medium + BA 1 mg/L+NAA 0 1～0 2 mg/L+GA 3 6～8 mg/L+CH 300 mg/L was good for the induction of bud; a medium +BA 1 mg/L+NAA 0 1 mg/L+GA 3 2 mg/L was suitable for propagation of the bud; and the basic medium+IBA 0 1 mg/L+NAA 0 5 mg/L was good for the induction of root更多还原