【摘要】 目的　研究麦冬总皂苷、人参总皂苷和生脉散的溶血作用及抗氧化剂对溶血的影响。方法　以氰化高铁血红蛋白法测定血红蛋白含量结合红细胞形态学观察。结果　人参总皂苷不溶血 ,麦冬总皂苷和生脉散溶血 ,溶血强度与皂苷浓度呈剂量依赖性。加入皂苷后红细胞有形态学改变。葡萄糖及抗氧化剂甘露醇和 Vit E能减轻皂苷的溶血作用。结论　皂苷溶血作用强弱与其所含皂苷的种类有关 ,并部分与增强红细胞膜的脂质过氧化有关。更多还原

【Abstract】 Object To study the hemolytic effect of total saponins from Ginseng (Gs), Radix Ophiopogonis (ROs) and Shengmaisan, a compound preparation of the two saponins, and the antihemolytic effect of antioxidants on hemolysis. Methods The percentage of hemolysis was indirectly calculated by measured cyanomethemoglobin, and morphological changes of red blood cells (RBCs) was examined by light microscope. Results Gs did not lead to hemolysis while both ROs and Shengmaisan induced lysis in a concentration dependent manner. RBCs deformation can be observed by light microscopic study. The hemolysis was partly inhibited by addition of glucose or antioxidants including mannitol and α-tocopherol. Conclusion The intensity of hemolytic effect varied with different saponins present in the compound preparation, and may be associated with the type of saponin and partly due to its ability to enhance lipid preoxidation of RBCs membrane.更多还原