【摘要】 目的　利用组织培养方法快速繁殖成米麻作为种麻 ,为寻找出一条简便可行的快繁天麻种麻的新途径提供理论依据。方法　天麻块茎或茎尖无菌离体培养 ,米麻块茎诱导培养基为 1/2 MS+6 - BA1mg/L+NAA0 .5mg/L +香蕉 5 0 mg/L ;箭麻茎尖诱导培养基为 1/2 MS+6 - BA 2 mg/L +NAA 0 .2 mg/L。结果　小米麻经 5 0 d无菌培养后开始生长出新米麻 ,70 d后原小米麻块茎上生长出多个分枝的新米麻 ;箭麻茎尖超过 140 d组织培养 ,茎尖分化、诱导形成原球茎 ,超过 16 0 d培养原球茎长大并开花。结论　天麻可以用组织培养方法快速繁殖成米麻作为种麻更多还原

【Abstract】 Object To develop a new method for the culturing of Gastrodia B1 in vitro, which may provide the theoretical basis of clonal propagation for its rapid reproduction Methods The small stem tubers and the stem buds were used as explants to culture in vitro under sterile conditions In the 1/2 MS medium containing 6 BA 1 mg/L, NAA 0 5 mg/L and banana 50 mg/L, the small stem tuber was induced to form protocorm In the 1/2 MS medium containing 6 BA 2 mg/L and NAA 0 2 mg/L, the stem bud was induced to form protocorm Results Each stem tuber formed a new protocorm within 50 d, which can be separated again to form rosette protocorm within 70 d The stem bud was cultured in vitro to form the protocorm within 140 d The protocorm bloomed within 160 d Conclusion The protocorm of G elata may be induced from the stem tuber and stem bud By subdividing these rosette protocorms a virtually indefinite clonal propagation can be achieved更多还原