【摘要】 目的 :研究脂多糖 (LPS)作用下的血管平滑肌细胞血红素加氧酶 - 1(HO - 1)基因的表达 ,并探讨HO/一氧化碳 (CO)调节通路在内毒素性血管调节功能障碍中的作用。方法 :10mg/L、30mg/L及 5 0mg/L的LPS与培养的大鼠主动脉平滑肌细胞 (VSMC)共同孵育 6h ,以及 10mg/LLPS与VSMC共同孵育 9h和 18h。分别观察细胞丙二醛 (MDA)含量及乳酸脱氢酶 (LDH)活性、台盼蓝染色法记录VSMC蓝染率的变化 ;Northern杂交测HO - 1mRNA表达。结果 :VSMCHO - 1mRNA表达随LPS浓度的增大而增加 ,LPS 5 0mg/L时HO - 1mRNA表达比对照高176 7% ;低剂量LPS(10mg/L)诱导VSMCHO - 1mRNA的表达量随诱导时间的延长而增加 ,18h组HO - 1mRNA的表达量比对照高 195 6 %。只有当LPS浓度增加至 5 0mg/L及 10mg/L孵育 18h时 ,细胞台盼蓝摄取率、MDA含量与LDH活性明显增加、细胞出现损伤。结论 :LPS可诱导VSMCHO - 1mRNA的表达且具有浓度依赖性和时间依赖性 ,HO/CO可能为介导内毒素性血管平滑肌功能调节障碍的重要通路更多还原

【Abstract】 AIM: To study the alterations of heme oxygenase-1 mRNA in vascular smooth muscle cells(VSMC) induced by lipopolysaccharide(LPS) and the role of heme oxygenase(HO)/carbon monoxide(CO)pathway in the disorders of regulation of cardiovascular system by LPS. METHODS: LPS (final concentrations 10 mg/L,30 mg/L and 50 mg/L) was added in cultured VSMCs for 6 h respectively or 10 mg/L LPS for 9 h and 18 h. MDA content, LDH release and the rate of trypan blue uptake of VSMC were measured. HO-1 mRNA expression was examined by Northern Blot. RESULTS: VSMC HO-1 mRNA expression was increased gradually with the increasing of LPS concentration. When final concentration of LPS was 50 mg/L, the HO-1 mRNA expression of VSMC was increased by 176.7% compared with control. When LPS final concentration was 10 mg/L, the HO-1 mRNA expression increased gradually along with the culture time. When cultured for 18 h, the HO-1 mRNA expression of VSMC was increased by 195.6% compared with control. Only at LPS 50 mg/L for 6 h and 10 mg/L for 18 h, the rate of trypan blue uptake,MDA content and LDH release were significantly increased. CONCLUSION: LPS can induce the HO-1 mRNA expression of VSMC and that were dose-dependent and time-dependent. The inducible HO may play an important role in the pathogenesis of vascular system under LPS. [更多还原