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转化CaMV基因Ⅵ芸薹属蔬菜植株抗病性鉴定及其遗传分析

Diagnosis and Genetic Analysis of Resistance to Cauliflower Mosaic Virus in Brassica Crops Which Was Transformed with CaMV Gene VI

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【作者】 巩振辉何玉科宋献军张广辉张桂华

【Author】 Gong Zhenhui 1,He Yuke 2,Song Xianjun 1,Zhang Guanghui 1,and Zhang Guihua 1 ( 1Horticulture College,Northwest Sci Tech University of Agriculture and Forestry,Yangling 712100; 2Shanghai Institute of Plant Physiology,The Chinese Academy of Sc

【机构】 西北农林科技大学园艺学院中国科学院上海植物生理研究所西北农林科技大学园艺学院 杨凌712100上海200032杨凌712100杨凌712100

【摘要】 以转化CaMV弱株系Bari 1基因Ⅵ的大白菜、菜薹、紫菜薹和花椰菜植株为试材 ,研究了CaMV弱株系Bari 1基因Ⅵ所提供的遗传工程交叉保护及其遗传规律。结果表明 ,在转化CaMV弱株系Bari 1基因Ⅵ的大白菜、菜薹、紫菜薹和花椰菜植株中 ,4 8.0 8%对CaMV强株系CABB BJI具有较强的抗性 ,5 1.92 %表现为敏感。抗性基因 (CaMVBari 1基因Ⅵ )在自交代 (S1)中大多数 (76 % )表现为典型的孟德尔单基因显性遗传 ,部分株系的抗性出现了15∶1,1∶1,1∶3和 1∶95的非孟德尔遗传现象

【Abstract】 The genetically engineered cross protection which was supplied by weak strain Bari 1 gene VI of cauliflower mosaic virus(CaMV),and its genetic regularity were studied with Brassica crops( Brassica campestris ssp. rapifera,B.campestris ssp. pekinensis,B.campestris var. parachinensis,B.campestris var. purpurea,B.juncea,B.oleracea var. botrytic )which were transformed with CaMV gene VI.The results showed that 48.08% of transformed Brassica plants was resistance to CaMV strain CABB BJI and 51.92% of them was susceptible to CaMV CABB BJI.The virus resistant gene(CaMV Bari 1 gene VI)in 76% of first selfed progeny presented typically Mendelian single dominance gene inheritance.The ratio of virus resistant to virus susceptible plants in some of first selfed progeny presented non Mendelian inheritance,such as,15∶1,1∶1,1∶3 and 1∶95.

【基金】 国家自然科学基金资助项目 (39770 5 2 2 ) ;教育部资助优秀年轻教师基金项目
  • 【文献出处】 园艺学报 ,Acta Horticulturae Sinica , 编辑部邮箱 ,2001年05期
  • 【分类号】S634
  • 【被引频次】19
  • 【下载频次】127
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