【摘要】 利用聚乳酸 ( PLA) /聚乙二醇 ( PEG) /聚乳酸 ( PLA)三嵌段共聚物 ( PELA)和乳化溶剂提取技术制备了包裹胰岛素的生物降解纳米微球 ,研究了囊材组成和分散剂对微球结构及胰岛素包封率、固载量和释放速率的影响 ,优化了制备微球的条件 .透射电镜和动态光散射测试表明 ,微球大小均匀 ,皆呈规整球形 ;粒径分布范围为 1 0 0～ 2 0 0 nm.微球对胰岛素的包封率和固载量分别达 87.6%和 1 7.5% .体外释放实验表明 ,微球可持续释药 1 0 d左右更多还原

【Abstract】 Nanospheres with entrapped insulin were prepared by a solvent extraction method with a series of copolymers of DL -lactide(PLA) and polyethylene glycol(PEG)(INS/PELA) where the PEG is in different molecular weight and content. The nanospheres as TEM shows have a smooth and spherical surface sized between 100~200 nm. The amount of insulin in nanospheres and the entrapment ratio achieved 17 5% and 87 6%, respectively. In vitro studies indicated that release of insulin from nanospheres could last over 10 days.更多还原