【摘要】 目的:了解紫外线照射库血后及其储存不同时期血液细胞免疫和细胞因子的变化情况,探讨紫外线照射库血的安全可行性。方法:采用成对血样本对照进行紫外线照射前后及其储存72小时,120小时后测定红细胞免疫功能,淋巴细胞亚群,淋巴细胞活性,淋巴细胞转化,白细胞介素2及其受体含量的变化。进行组间比较和不同储存时期比较。结果:①紫外线照射库血,对红细胞免疫功能,淋巴细胞亚群及淋巴细胞活性,白细胞介素-2及其受体无影响;②紫外线可抑制淋巴细胞的增殖转化;③库血储存时间的改变,细胞免疫及细胞因子均出现显著变化。结论:体外紫外线照射血液,可抑制淋巴细胞增殖转化,而不引起其他细胞免疫功能的变化。储存时间的改变会引起其显著变化。更多还原

【Abstract】 Objectives:To understand changes of cell immune function and cytokine in banked blood during different storage pe-riods post ultraviolet irradiation and to explore the safety and feasibility of ultraviolet on banked blood.Methods:paired blood samples are used in contrast.Changes of erythrocyte immune functions,lymphocyte subgroup.Lymphocyte activity,lymphocyte transformation,interleukolinⅡand the contest of its receptor have been tasted72h,120h pre and post ultraviolet irradiation separately.Comparison is made between groups and within one group.Results:①There is no change erythrocyte immune func-tions lymphocytes subgroup.Lymphocyte activity,interleukolinⅡand the contest of its receptor after ultraviolet irradiation;②ul-traviolet irradiation can inhibit lymphocyte transformation and decrease response to PHA;③obvioua change occurs to erythrocyte immune functions,lymphocyte subgroup.Lymphocyte activity,interleukolinⅡand the contest of its receptor according to different storage periods.Conclusions:ultraviolet irradiation will cause no changes to erythrocyte immune function,lymphocyte subgroup,interleukolinⅡand its receptor while different storage period will result in change to those issues.But its can inhibit lymphocyte transformation and decrease response to PHA.更多还原