【摘要】 目的 :建立水溶性药物呱仑酸钠血药浓度的高效液相色谱分析方法。方法 :以对二甲氨基苯甲醛为内标 ,在比较了固相萃取、离子对萃取及沉淀蛋白 -脱水法等 3种预处理方法的基础上 ,采用沉淀蛋白 -脱水法预处理兔血浆样品 ,分析柱为 μBondapakC18柱 (2 5 0mm× 4 6mm ,10 μm) ,流动相为 pH 7 0的 0 0 1mol·L-1磷酸盐缓冲液 -甲醇 (5 0∶5 0 ) ,流速1mL·min-1,在 2 93nm波长处检测 ,按内标法定量。结果 :血药浓度线性范围为 0 1～ 10 0mg·L-1(r=0 9997) ,最低检测浓度为 0 0 2mg·L-1(S/N >3) ,萃取回收率在 79 5 %～ 86 4% (n =5 ) ,分析方法回收率在 93 1%～ 95 5 % (n =5 ) ,日内和日间RSD分别为 1 2 %～ 1 8%和 1 9%～ 3 1% (n =5 )。结论 :本法简便、准确、精密度高、重现性好 ,适用于呱仑酸钠的体内研究更多还原

【Abstract】 Objective:To establish HPLC method for determination of water-soluble drug sodium gualenate(SGL)in rabbit plasma. Methods:In the experiments,three clean-up techniques of rabbit plasma samples(ion-pair extraction,solid-phase extraction and protein precipitation-dehydration method) were investigated and compared comprehensively,and protein precipitation-dehydration was chosen to pretreat plasma samples before HPLC analysis.Using p -(dimethylamino)-benzaldehyde as an internal standard(IS),the SGL and IS were separated on the μBondapak C 18 column(250 mm×4 6 mm,10 μm)with 0 01 mol·L -1 phosphate buffer of pH 7 0-methanol(50∶50) as the mobile phase,a flow rate of 1 mL·min -1 and detected at 293 nm.Results:The linear range of calibration curve was within drug plasma concentrations of 0 1～10 0 mg·L -1 ( r =0 999 7),the detection limit was 0 02 mg·L -1 ( S/N >3),the extraction recovery was 79 5%～86 4%( n =5),the analytical recovery was 93 1%～95 5%( n =5),the within-day RSD and between-day RSD were 1 2%～1 8% and 1 9%～3 1%( n =5)respectively.Conclusions:The method possesses merits of simplicity,sensitivity,good accuracy,high precision,and fine reproducibility,which was suitable for the study of SGL in vivo.更多还原