【摘要】 目的 :建立高效液相色谱柱前衍生化结合柱切换技术测定血浆中卡托普利浓度的方法。方法 :血浆中卡托普利与对溴苯甲酰甲基溴发生衍生化反应后 ,分别加入等体积的丙酮和水 ,离心后取上清液进样 ,以HPLC柱切换进行分离测定。结果 :线性范围为 2 0～ 10 0 0ng·mL-1,净化回收率为 94 7%～ 96 2 % ,方法回收率为 98 1%～ 10 3 8% ,日内和日间检测RSD分别小于 2 8%和 4 2 %。结论 :方法简便灵敏 ,为卡托普利人体药代动力学研究及临床药物监测提供了方法更多还原

【Abstract】 Objective:To establish a method using HPLC with derivatization and column switching for determination of captopril(Cap) in human plasma.Methods:Cap in plasma was derivatized with p -bromophenacyl bromide ( p -BPB),then acetone and water were added into plasma with same volume,respectively.After centrifugalizing,the derivatization products,Cap- p \|BPB,in plasma samples were determined by HPLC with column switching.Results:A linearity range of 20～1 000 ng·mL\+\{-1\} was obtained.The clean-up recoveries were from 94 7% to 96 2%,and the method recoveries were 98 1%～103 8%.The RSD of within-day and between-day were less than 2 8% and 4 2%,respectively.Conclusion:The method is simple,sensitive and suitable for pharmacokinetics studies and clinical detection of Cap.更多还原