【摘要】 目的　确定鼠脑细胞体外培养的适宜生长基质。方法　以Balb/C鼠尾胶原与大鼠尾胶原作为生长基质 ,培养新生Balb/C鼠脑细胞。结果　Balb/C鼠尾胶原有利于神经元的生存、生长与分化 ,而大鼠尾胶原则利于星形胶质细胞的生存、生长、分化与增殖。结论　 2种鼠尾胶原均为培养鼠脑细胞适宜的生长基质 ,培养 1个月左右宜用Balb/C鼠尾胶原而长期培养则宜选择大鼠尾胶原更多还原

【Abstract】 Objective To determine the suitable growth substrate for brain cells in culture. Methods Two kinds of collagen from Balb/C mouse tail and rat tail respectively as growth substrate was applied to culturing newborn Balb/C mouse brain cells. Results Balb/C mouse tail collagen was suitable to the survival, growth, differentiation of neurons, whereas rat tail collagen those of astrocytes. Conclusion Two kinds of collagen are suitable as growth substrate of neuron culture. Balb/C mouse tail collagen may be applied as growth substrate for short term cultures, whereas rat tail collagen may be adopted for long term cultures.更多还原