【摘要】 目的 :本实验采用高效液相色谱法 ,以离体小白鼠腹部皮肤为透皮屏障 ,比较了四种双氯灭痛软膏剂的透皮效果。方法 :实验用法国Gilson公司的HPLC仪 ,以C18为固定相 ,甲醇 水 (含HAc 0 .36 % ) =80∶2 0为流动相 ,紫外检测波长 2 80nm。平均回收率为 99.5 9% ,RSD <3.2 8% ,检测限为 1.0 μg/ml。 结果 :1号样品透皮率最高 ,3号与 1号相比较透皮吸收无显著性差异(P >0 .0 5 ) ,2号或 4号分别与 1号相比 ,其透皮吸收均有显著性差异 (P <0 .0 5 )。结论 :1号样品值得进一步开发。更多还原

【Abstract】 OBJECTIVE:A HPLC method was established to determine the concentration of diclofenac sodium in transdermal absorption liquid.METHOD:A YWG C 18 (10μm) colum was used with a mobile phase of CH 3OH H 2O(including HAc 0.36%)(80∶20) at a flow rate of 1.0ml/min.UV detector was set at 280nm.The average recovery was 99.59% and the RSD were less than 3.28%.RESULTS:The No.1 and No.3 ointments had similar good effect on transdermal absorption(P>0.05) while the percutaneous release rate of No.1 was higher.The No.1 was absorbed more quickly than the No.2 and No.4(P<0.05).CONCLUSION:The No.1 ointment is valuable to be further developed.更多还原