【摘要】 本实验在 67只豚鼠过敏性休克模型上 ,观察不同强度或频率电针“内关”穴对过敏性休克豚鼠低血压反应的影响 ,并用放免法测定下丘脑、垂体前后叶催产素 (OT)的含量 ,试图初步探讨电针“内关”穴抗过敏性休克的作用机理。结果提示 ,电针“内关”穴有明显而快速的抗过敏性休克的作用。电针频率相同时 ,高强度电针“内关”穴的升压效应比低强度的好 ;电针强度不变时 ,高频电针“内关”穴的抗过敏性休克作用显著优于低频者。电针“内关”穴抗过敏性休克的作用均有OT介导。高强度电针时OT似乎是通过促进垂体前叶ACTH等激素释放实现的 ;而高频电针的升压效应可能是促进了下丘脑OT的释放所致。更多还原

【Abstract】 Objective: To study the therapeutic effect of electroacupuncture (EA) on alergic shock in guinea pigs. Methods: Experiments were performed on 60 anaphylactic shock model guinea pigs (anesthetized by intra peritoneal injection of pentobarbital sodium 30 mg/kg) who were randomly divided into 60 ～70 Hz plus 1 V (n=7), 30 ～ 40 Hz plus 3 V (n=7) and 30～40 Hz plus 1 V (n=7) groups. The 3 groups were further divided into EA of Neiguan (PC 6) and Non acupoint subgroups. Alergic shock was produced by intra peritoneal and intravenous injection of ovokeratin 10 μg/kg and 0.26～0.28 μg/kg respectively in different days. Mean blood pressure (MAP) was determined via right carotid catheter. Bilateral “Neiguan” (PC 6) were stimulated with frequency 60～70 Hz, 30～40 Hz, voltage 1 V and 3 V and stimulating duration of 30 min. The contents of oxytocin(OT) in hypothalamus,anteropituitary and postpituitary tissues were measured by radioimmunoassay. Results: The results suggested that EA at “Neiguan” point could produce a significant and rapid effect of antianaphylactic shock in comparison with non acupoint group. Under the same frequency, the pressor effect of EA at “Neiguan” point at higher stimulating intensity was significantly better than that of low intensity. Similarly, under the same intensity, the effect of higher frequency was considerably better than that of low frequency in anti anaphylactic action. After EA stimulation at frequency of 30～40 Hz plus voltage of 3 V and 60～70 Hz plus 1 V, OT contents decreased significantly in the hypothalamus and posteropituitay or increased considerably in anteropituitary tissue in comparison with antianaphylactic shock model group (n=7, P< 0.01, 0.05); while after EA at frequency 30～40 Hz plus voltage 1 V, OT contents had slight increase in hypothalamus and postpituitary or slight increase in anteropituitary but without statistical difference in comparison with shock model group. When EA at a fixed frequency (30～40 Hz) or at a fixed stimulating strength (1 V), OT values of voltage 3 V (n=7) and 60～70 Hz groups increased significantly in anteropituitary or decreased significantly in hypothalamus in comparison with voltage of 1 V or 30～40 Hz groups separately (P<0.05). It indicates that the effect of antianaphylactic shock of EA at “Neiguan” point may be mediated by OT. OT seems to be involved in the pressor response by inducing the release of ACTH from anteropituitary under higher intensity EA; while OT released from hypothalamus might be responsible for the pressor response under higher frequency EA.Conclusion: EA possesses a good pressor action in alergic shock guinea pigs, which may be mediated by OT in the hypothalamus.更多还原