【摘要】 筛选出适宜小麦转化的优良基因型扬麦 15 8和扬麦 10号。以扬麦 15 8幼胚愈伤组织为受体 ,利用基因枪介导法将含有小麦黄花叶病毒复制酶基因 (WYMV- N ib8)的 pubi Nib8质粒转入了小麦。T0 代经 PCR扩增分析 ,获得了 14株含 WYMV- N ib8基因的转化植株 ,转化率为 0 .4 3%。T1、T2 代跟踪分子检测和 T2 代田间病毒抗性鉴定结果表明 ,获得了 4个抗 WYMV的转基因株系更多还原

【Abstract】 Two wheat genotypes with high tissue culture ability, Yangmai 158 an d Yangmai 10, were screened from ten commercial varieties for the following tran sformation. Then, the seven days immature embryo calli of Yangmai 158 used as re ceptor of Nib8 target gene, which was isolated from wheat yellow mosaic viru s, w ere bombarded by biolistic particle. 237 resistant plantlets to 3～5 mg/L B ia laphos were achieved on the selection medium. By PCR analysis in the T 0 genera tion, fourteen transgenic plants were identified with a transformation frequency of 0.43%. Four transgenic wheat lines strongly resistant to the diseas e were obtained combining the molecular test of T 1, T 2 and WYMV field test o f T 2.更多还原