【摘要】 目的 研究HIV-1相关的等位基因CCR5△32、CCR5m303、CCR2b和SDF1在艾滋病病毒感染人群中的分布,为深入分析这些遗传突变在HIV-1感染、艾滋病发病进程中的作用提供理论依据。方法 收集深圳地区近年来艾滋病病毒感染者的血液标本共91份,提取基因组DNA。经PCR或PCR-RFLP分析,计算遗传突变频率、纯合子和杂合子的构成比例。采用x2检验或t检验进行群体分布的拟合度和差异的显著性分析。结果 在所有被检测的91例HIV-1感染者的个体中,未发现有CCR5△32和CCR5m303的遗传突变,CCR2b-64I和SDF1-3＇A的突变频率较高,分别为21.43％和25.82％。与中国普通汉族人的结果相近。结论 研究结果提示深圳地区的艾滋病病毒感染者本身对HIV-1病原体可能具有较大的遗传易感性。由于他们具有较高的CCR2b-64I和SDF1-3＇A突变频率,后者在艾滋病发病过程中的影响值得进一步研究。更多还原

【Abstract】 Objective To evaluate polymorphisms of CCR5△32, CCR5m303, CCR2b-64I and SDFl-3’ A alleles in HIV-1 carriers in Shenzhen City. Methods Genomic DNA was purified from 91 blood samples of HIV - 1 carriers by using QIAamp DNA Blood Mini Kit and identified by PCR or PCR - RFLP assays. Results The study showed that the mutation frequency of CCR5△32,CCR5m303,CCR2b-641 and SDFl-3’A alleles was 0.0% , 0% , 21.43% and 25.82%, respectively in HIV-1 carriers, and was similar to that in the Chinese population of Han ethnic Origin;the latter possess much lower frequency of CCR5△32 and CCR5m303, and higher frequency of CCR2b - 64I and SDF1 - 3’ A alleles. Conclusion The results suggest that HIV-1 carriers in Shenzhen City of China may be genetically susceptalbe to HIV-1, and the significance of higher frequencies of CCR2b-64I and SDFl - 3’A alleles need to be further evaluated.更多还原