【摘要】 选择 12 2树脂用于丙酮酸发酵液的脱色 ,在发酵液丙酮酸质量浓度为 60 g/L、pH 3.0、脱色空间流速为 1.8mL/(mL·h)的条件下可取得较好的脱色效果 ,丙酮酸损失率低于 6% .从 8种碱性阴离子交换树脂中筛选出 330弱碱性阴离子树脂 (氯型 ) ,用于从发酵液中提取丙酮酸 .考察了不同操作条件对固定床离子交换效果的影响 ,上柱料液 pH的变化对流出曲线的形状影响不大 .当上柱料液的 pH和丙酮酸质量浓度分别控制在 4 .0和 4 0 g/L时 ,可以获得较高的有效体积交换容量 (分别为 0 .534mol/L树脂和 0 .530mol/L树脂 ) .实验范围内体积流量对交换过程的影响不大 ,在较低的离子交换空间 ( 0 .4 4mL/(mL·h) )下有效体积交换容量较高 ( 0 .60 4mol/L树脂 ) .考察了固定床洗脱工艺条件对洗脱效果的影响 .当洗脱剂盐酸的浓度为 2mol/L、洗脱空间流速控制在 0 .4 4mL/(mL·h)左右时洗脱效果较好 .采用较佳的工艺条件进行固定床单柱操作 ,实验得到离子交换单元的丙酮酸收率为 97.0 % .更多还原

【Abstract】 resin was chosen to decolor the pyruvate fermentation broth. Excellent decoloration result was achieved under the following conditions: pH 3.0, 60 g/L pyruvate concentration of broth, 1.8 mL/(mL·h). 330 weakly basic anion resin was selected for its superior separation performance to purify pyruvate. In the fixed bed ion exchange operation, high total exchange capacity was obtained when pH and pyruvate concentration of the influent were kept around 4.0 and 40 g/L, respectively. High effective exchange capacity (0.604 mol/L) was achieved under lower flow rate of influent. Elution experiments demonstrated that 2 mol/L hydrochloric acid and lower flow rate (0.44 mL/ (mL·h) ) benefit high elution efficiency. Under the optimal conditions, the recovery percent of pyruvate from fermentation broth achieved 97.0% in ion exchange operation unit.更多还原