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巨大芽孢杆菌(Bacillus megaterium)突变株Bn,B5的生物学特性及在Vc二步发酵中的伴生作用

BIOLOGICAL CHARACTERISTICS OF Bacillus megaterium MUTANTS BN AND B5 AND THEIR COMPONENT EFFICIENCY IN VC TWO-STEP FERMENTATION

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【作者】 张舟冯树谢占武曹桂环张忠泽

【Author】 Zhang Zhou et al. (Inst. of Applied Ecology, Academia Sinica, Shenyang 110015)

【机构】 中国科学院沈阳应用生态研究所!辽宁沈阳110015东北制药总厂!辽宁沈阳110026

【摘要】 对选出的巨大芽孢杆菌突变株Bn ,B5进行了生物学特性及发酵条件的研究 ,发现它们具耐低 pH和抗高浓 2KGA特性。可促进氧化葡萄糖酸杆菌生长 ,使其延迟期缩短 ,产酸增加。适宜的通气量下 ,摇瓶糖酸转化率提高 10 %~ 14% ;当发酵 pH为 6 .2~ 6 .6时 ,转化率提高 2 0 %~ 30 %

【Abstract】 Biological characteristics and fermentation conditions of Bacillus megaterium mutants Bn and B5 were studied. The optimal pH for Bn and B5 growth was at 6.0-8.0. They graw well in the medium with 3% 2KGA and at pH6.2. The conversion rates of Gluconobacter oxydans with them from L sorbose to 2KGA were increased by 10%-14% under suitable aeration and 20%-30% at fermentation pH at 6.2-6.6 as compared with CK strain. The growth of Gluconobacter oxydans was promoted by Bn and B5 and shortened its delay phase and increased its acid production.

【基金】 辽宁省科委重大科技攻关项目!(972 2 10 11);沈阳市科委重大科技攻关项目!(96 2 1130 6 0 1)
  • 【文献出处】 微生物学杂志 ,Journal of Microbiology , 编辑部邮箱 ,2001年01期
  • 【分类号】Q939
  • 【被引频次】10
  • 【下载频次】168
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