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特效黄杆菌对蒽菲芘的降解性能研究
THE STUDY OF DEGRADATION OF ANTHRACENE,PHENANTHRENE AND PYRENE BY EFFECTIVE FLAVOBACTERIUM
【摘要】 采用定时、定量、逐步提高驯化所用碳源物浓度的方法 ,以萘为唯一碳源驯化长期被焦化废水污染的泥土浸出液 ,7周后 ,平板划线分离出两株黄杆菌FCN1及FCN2。并对这两株菌降解多环芳烃的特性及无机离子对反应的刺激作用进行了研究。结果表明 ,FCN1及FCN2能降解转化蒽、菲、芘。加入FCN1 ,反应 1 0h后 ,蒽、菲、芘去除率分别为84%、69%、 80 % ,而加入FCN2 ,各物质的去除率分别为 76%、 40 %、 71 %。反应进行1 0 6h,FCN1对蒽、菲、芘所产生的总有机碳 (TOC)的去除率分别为 70 %、 5 4 %、 69% ,而FCN2对相应物的TOC去除率分别为 63%、 5 0 %、 46%。Fe3+、Mg2 +的加入对FCN1降解多环芳烃有促进作用
【Abstract】 Two effective flavobacterium strains FCN1 and FCN2 were isolated from the sludge which had been contaminated by coke-plant waste water for a long time.Before isolation,the naphthalene was used to cultivate such sludge for seven weeks as the sole carbon source and its concentration adding to the cultivating system was increased up to 50mg/L gradually.The polycyclic aromatic hydrocarbon degrading characteristics of our isolates themselves and with inorganic ions was studied respectively.The testing results show that our two isolates can transform and degrade anthracene,phenanthrene and pyrene.After 10 hours reaction,the removing rates of anthracene,phenanthrene and pyrene by FCN1 were 84%,69%,80% and by FCN2 were 76%,40%,71% respectively.After 106 hours,the removing rates of TOC caused by anthracene,phenanthrene and pyrene were 70%,54%,69% by FCN1 and 63%,50%,46% by FCN2.The Fe 3+ and Mg 2+ can accelerate the degrading reaction of FCN1.
【Key words】 Flavobacterium; Biodegradation; Polycyclic aromatic hydrocarbons;
- 【文献出处】 微生物学通报 ,Microbiology , 编辑部邮箱 ,2001年05期
- 【分类号】X172
- 【被引频次】25
- 【下载频次】250