【摘要】 电子供体连二亚硫酸钠、甲基紫精及电子受体亚甲蓝均能强烈抑制棕色固氮菌表达固氮活性 ,并引起该菌的抗氨阻遏能力减弱。适当提高氧压 ,能提高菌体的固氮活性近1 5 % ,但过高的氧分压反而抑制菌体的固氮活性。此外 ,提高氢分压能降低棕色固氮菌菌体内的还原电位 ,从而达到提高菌体抗氨阻遏能力的效果。更多还原

【Abstract】 Electronic donors dithionite as well as m et hyl viologen and it receptor methylene blue strongly inhibited activity of nitro gen fixation that Azotobacter vinelandii expressed,which ca used capacity of ammonia-resistant weaken greatly.About 15% of total activity f rom the whole cell were risen by increasing suitable oxygen atmosphere.Opposite, t he activity was inhibited by increasing over concentration of O 2 further.The r eduction potential in the inside environment from Azotobacter vinelan dii whole cell shifted to negative by increasing hydrogen pressure,whi ch resulted in its capacity of ammonia-resistant rose.更多还原