【摘要】 目的　为了快速克隆ＰＣＲ产物，本实验构建了简单、方便的 ＴＡ载体。方法 ＰＢｌｕｅｓｃｒｉｐｔ ＳＫ（－）经 ＥｃｏＲ Ｖ酶切形成平端切口，在ＴａｑＥ的催化下加一个ｄＴＭＰ形成３’端带有Ｔ的粘端载体，在Ｔ４ＤＮＡ连接酶作用下，将ＰＣＲ产物互补连接于ＴＡ载体上，重组质粒转化细菌，碱裂解法提取重组质粒ＤＮＡ，酶切及电泳鉴定。结果ＴＡ载体成功地克隆了ＰＣＲ产物。结论构建的ＴＡ载体简单、方便，可以克隆任何ＰＣＲ产物。更多还原

【Abstract】 Objective: To clone polyrnerase chain reaction products rapidly, a convenient and practical TA-vector was constructed. Methods The pBluescript SK(-) was digested by EcoRV and a flat end was developed. A dTMP was added to form a sticky end with a T in 3 end. The PCR products were ligated with TA-vector by T4 DNA ligating enzyme. The recombinant plasmid was transferred to bacteria and distilled by alkaline solution method. The results were confirmed by reaction endonuclease digestion and electrophoresis. Results The polymerase chain reaction products were cloned by TA-vector. exclusion The TA-vector is a universal and practical structure for any PCR products.更多还原