【摘要】 用 PCR方法获得编码人组织型纤溶酶原激活物 (tissure- type plasminogen activator,t- PA) C端 P区肽段的 DNA,并经测序证实 ,长 810 bp,含有全部的 t- PA的丝氨酸蛋白酶编码序列 .将这段序列克隆到表达载体p QE30中转化大肠杆菌 JM10 9菌株 ,经 SDS- PAGE检测 :转化菌株中表达出分子量约 2 9× 10 3的蛋白 ,用纤维平板法测定激活纤溶活性 ,结果表明表达的 t- PA蛋白 C端 P区具有很强的激活纤溶蛋白酶原的活性 .证实 t- PA蛋白 C端 P区的酶结构区的功能是独立的 ,其生物活性不受其它区域影响更多还原

【Abstract】 The DNA fragment encoding t-PA P region protein was obtained by PCR,using plasmid pTAhtpa(F) harborong the full length t-PA cDNA as template. This P region fragmemt was cloned into pQE30 and transformed to JM109 strain.P region proteins fused to 6 His peptides at its N terminus with molecular weight of 29×10 3 were expressed in JM109 induced by IPTG.The expressed protein were purified by Ni-NTA-agarose and were studied its activity by fibrin plate activity assay(FPAA).The results show that the function of t-PA P region is indepent for other regions of t-PA. and it has significant activity to stimulate plasminogen.更多还原