【摘要】 目的 :调查我院感染科1998年168株致病菌产β-内酰胺酶、超广谱β-内酰胺酶及对多种抗生素的耐药情况 ,以指导临床用药。方法 :采用Nitrocefin纸片法检测β-内酰胺酶 ,双纸片法检测超广谱酶 ,药敏纸片检测细菌耐药情况。结果 :革兰氏阴性杆菌约91.9 % β-内酰胺酶阳性 ,葡萄球菌66.7 %阳性 ,111株革兰氏阴性杆菌中7株ESBLs阳性 ,均为肠杆菌科菌 ;环丙沙星耐药率 (尤其对大肠杆菌 )逐年上升 ,泰能、头孢他定对革兰氏阴性杆菌耐药率最低 ,万古霉素和替考拉宁对革兰氏阳性球菌耐药率最低。结论 :临床致病菌大部分产β-内酰胺酶 ,约10 %肠杆菌科细菌产超广谱酶 ,泰能和万古霉素分别对革兰氏阴性杆菌和革兰氏阳性球菌耐药率最低更多还原

【Abstract】 Objective:To guide proper antibiotic use, the clinical isolating strains on detecting β-lactamase,extended-spectrum β-lactamase and multiple resistance were studied. Methods: The disks containing Nitrocefin were used to detect β-lactamase .Double-disk assays were used to detect ESBLs. Disk susceptibility test was done according to the standard methods. Results: 91.9% of gram-negative bacilli and 66.7%of staphylococci Spp were β-lactamase producing strains. 7/111 of gram-negative bacilli were ESBLs, which all are enterobacter Spp. The resistant rate of Ciprofloxacin to isolating bacteria gradually raised year by year, special to E.Coli. The resistant rate of gram-negative bacilli to imipenem and cefatazidime was lower than to other drugs. Also, the resistant rate of gram-positive cocculi to vancomycin and teicoplanin is lower than to other commonly used antibiotics. Conclusion: Only do a few of strains were not β-lactamase-produced strains.10%strains of enterobacteriaceae produced ESBLs. The resistant rate of gram-negative bacilli to Lmipenem and the resistant rate of gram-positive cocculi to vancomaycin were the lowest in all studying antibiotics.更多还原