【摘要】 目的　寻找胶质母细胞瘤 (GBM) 10号染色体上可能存在肿瘤抑制基因的杂合性丢失 (L OH )区域 ,为发现和定位肿瘤抑制基因 (TSG)提供线索和依据。方法　应用聚合酶链反应 (PCR)方法 ,采用荧光标记的引物和先进的 377型 DNA序列自动分析仪 ,分析了 2 1例 GBM10号染色体上 2 0个微卫星多态性标记的 L OH。结果　在85 .7% (18/ 2 1例 ) GBM的 10号染色体上观察到 L OH,在 5 7.7% (16 2 / 2 81)可提供信息位点上存在 L OH。 10 q的L OH率高于 10 p,分别是 81.0 % (17/ 2 1)、6 6 .7% (14/ 2 1)。在下列位点或区域检测到较高的 L OH率 (>6 0 % ) :10 q2 2 .3～ 2 3.3上的 D10 s185～ D10 s192间区域 ,10 p14～ 15 .1上的 D10 s5 91～ D10 s2 49间区域 ,10 q2 4.2～ 2 6 .3上的 D10 s16 93～ D10 s2 12间区域 ,10 p12 .2～ 14上的 D10 s5 47位点 ,10 q2 1.3上的 D10 s5 37位点。结论　 10号染色体可能在 GBM的分子水平发病机制中发挥着重要作用 ,它上面的多个染色体区域可能存在与 GBM相关的多个TSG。更多还原

【Abstract】 Objective To locate the deletion areas probably harboring tumor suppressor genes (TSGs) in glioblastoma(GBM).Methods Twenty loci on chromosome 10 were examined to detect LOH in 21 cases of glioblastoma (GBM).PCR based microsatellite polymorphism analyses were performed to detect loss of heterozygosity (LOH)on chromosome 10,fluorescence-labeled primers and Perkin Elmer 377 DNA Sequencer were applied.Results Among all informative cases of GBM,85.7% displayed LOH on chromosome 10,81.0% of displayed LOH on 10q and 66.7% on 10p.57.7% of informative loci showed LOH in our series,in which the higher frequent LOH(>60%) were observed in the following chromosomal region or loci:from D10s185 to D10s192 on 10q22.3-23.3,D10s519 to D10s249 on 10p14-15.1,D10s1693 to D10s212 on 10q24.2- 26.3 ,D10s547 on 10p12.2-14,D10s537 on 10q21.3.Conclusions Loss of genetic material on chromosome 10 may play an important role on the molecular pathogenesis of GBM.The chromosomal regions from loci D10s185 to D10s192 on 10q 22.3 -23.3,D10s591 to D10s249 on 10p14-15.1,D10s1693 to D10s212 on 10q24.2-26.3,and at loci D10s547 on 10p12.2-14 and D10s537 on 10q21.3 may harbor novel tumor suppressor genes associated with GBM.更多还原