【摘要】 目的　探讨去铁胺 (DFO)对HL 6 0细胞凋亡的诱导作用及对c myc基因表达的影响。 方法　HL 6 0细胞与不同浓度的去铁胺培养 6、12、2 4、48h。分别测定细胞活力 ,观察形态学变化 ,用流式细胞学检测和DNA凝胶电泳等观察细胞凋亡及免疫组化方法检测c myc基因表达等指标。结果　DFO可降低HL 6 0细胞活力、抑制HL 6 0细胞增殖、诱导其凋亡 ,并使c myc基因表达增加 ,其作用随培养时间延长及DFO浓度增加而明显。结论　DFO可影响HL 6 0细胞DNA的合成 ,诱导其凋亡 ,c myc的表达增加可能是铁剥夺诱导HL 6 0细胞凋亡的机制之一。DFO可作为治疗或辅助治疗白血病的药物用于临床 ,将有重要的临床实用价值更多还原

【Abstract】 Objective To explore the apoptosis induced by deferrioxamine(DFO) in HL 60 cells and influence on c myc expression.Methods HL 60 cells were incubated for 6, 12, and 24 hours with different concentrations of DFO at 37 ℃ in RPMI 1640 containing 10 % heat inactived fetal bovine serum in water saturated 5 % CO 2 incubator. The indices were detected including living cells count and viability, apoptosis by morphologial study, DNA agarose gel electrophoresis, and flowcytometry (FCM) assay, expression of c myc by immunocytochemistry.Results When HL 60 cells were incubated with different concentration of DFO, APO % showed dose time dependent and was much higher than that of control group (P<0.01). Apoptotic peak appeared slowly even if at higher concentration of DFO group. FCM assay showed that apoptosis of HL 60 cells occurred mainly in the S phase. Living cell count and cell viability declined with the increase of DFO. The expression of c myc showed dose time dependent and was much higher than that of control group (P<0.05) Expression of c myc in HL 60 cells was higher in DFO groups than control group.Conclusions DFO could inhibited proliferation and induced apoptosis in HL 60 cells. Upregulation of c myc expression may result in HL 60 cells apoptosis. It suggests that the DFO may play a role in the treatment of leukemia.更多还原